HANDBOOK OF IMMUNOHISTOCHEMISTRY. FREQUENTLY ASKED QUESTIONS

DESCRIPTION

As in thesecond edition, the third edition of Handbook of PracticalImmunohistochemistry Frequently Asked Questions is written in a questionand answer (Q&A) format and intended to be a practical, user-friendly,quick reference for information related to using the most up-to-dateimmunohistochemistry and in situ hybridization in clinical diagnosis. The newedition demonstrates a significant revision and improvement over the secondedition. Five new chapters have been added that cover the following: 1)Immunohistochemistry: Leicas perspective; 2) Immunohistochemistry: Maixin perspective; 3) RNA in situ hybridization:Applications in anatomic pathology; 4) Applications of rapidimmunohistochemistry on frozen tissue sections during intraoperative pathologicdiagnosis; and 5) Cutaneous lymphomas. In addition to extensive additions andchanges, over 150 new questions and answers were added throughout this new edition.All chapters have been updated to include relevant new questions, new markers,more refined IHC panels, representative pictures, and current references. An extensive set of high-quality color pictures anddiagnostic algorithms, if available, is included in each chapter to illustratesome of the key antibodies, including many recently discovered andsubstantiated antibodies used in that chapter.
Written by experts in the field, Handbook of PracticalImmunohistochemistry Third Edition is a comprehensive and practical guidefor surgical pathologists, pathology residents and fellows, cytopathologists,and cytotechnologists.

TABLE OF CONTENTS

• Immunohistochemistry Quality Management and Regulation

Jeffrey W. Prichard

• Standardization of Diagnostic Immunohistochemistry

Fan Lin, Jianhui Shi

• Automated Immunohistochemistry Overview

Jeffrey W. Prichard, Angela K. Bitting

• Immunohistochemistry: An Agilent Perspective

Ole F. Rasmussen, Lars Rudbeck

• The Leica Biosystems Perspective: From Excision to ImagingEvery Step Is Critical

Douglas Coveney, Mandy Lindsay, Claire Kentler, Kellie Madigan

• Immunohistochemistry: Maixin Perspective

Xiaoya Wang, QixinLin, Yulin Xiong

• Immunohistochemistry: Roche Tissue Diagnostics Perspective

Hiroaki Nitta, Mark D. Robida, Nate Polaske

• Applications of Rapid Immunohistochemistry on Frozen Tissue Sections During Intraoperative Pathological Diagnoses

Evans C. Bailey, Song Q. Zhao

• RNA In Situ Hybridization: Applications in Anatomic Pathology

Fan Lin, Jeffrey Kim, Robert Monroe

• Overview of Immunohistochemistry Assessment of Cancer-Related Predictive Biomarkers and Common Genetic Alterations

Zongming Eric Chen, Yi Ding, Fan Lin

• Tissue Microarray

Myra L.Wilkerson, Stephen Hewitt

• Unknown Primary/Undifferentiated Neoplasms

Fan Lin, HaiyanLiu

• Exfoliative Cytology and Effusions

Renee Frank, Steven C. Meschter

• Breast

HaiyanLiu, Chelsea R. Mehr

• Predictive Biomarkers in Breast Cancer: ER, PR, and HER-2/NEU

Jeffrey W. Prichard, Chelsea R. Mehr, David G.Hicks, Elizabeth Hammond

• Central Nerve System

Syed A. Jaffar Kazmi, Conrad Schuerch

• Thyroid, Parathyroid, and Adrenal Glands

Haiyan Liu, Myra L. Wilkerson, Fan Lin

• Salivary Glands and Head and Neck

William Quinones, Conrad Schuerch, Shaobo Zhu

• Pleuropulmonary and Mediastinal Neoplasms

HongbingDeng, Kai Zhang

• Uterus

Thomas J. Grifone, Christopher P. Crum

• Ovary

Jeffrey W. Prichard

• Prostate Gland

HaiyanLiu, Fan Lin, Qihui Jim Zhai

• Urinary Bladder and Urachus

Myra L.Wilkerson, Liang Cheng

• Kidney

Fan Lin, XimingJ. Yang

• Testis and Paratesticular Tissues

Myra L.Wilkerson, Liang Cheng

• Pancreas and Ampulla

Fan Lin, HanlinL. Wang

• Liver, Bile Ducts, and Gallbladder

Jianhong Li, ZongmingEric Chen, Fan Lin

• Upper Gastrointestinal Tract

JinhongLi, Fan Lin

• Lower Gastrointestinal Tract and Microsatellite Instability (MSI)

Guoli Chen, Jianhong Li, ZongmingEric Chen, Jinhong Li, Fan Lin

• Soft Tissue and Bone Tumors

George Lin, ShaoboZhu

• Lymph Node

XiaohongMary Zhang, Yi Ding

• Bone Marrow

Michelle L. Grant, Xiaohong Mary Zhang

• Cutaneous Lymphomas

Jacqueline M. Junkins-Hopkins

• Infectious Diseases

Dirk M. Elston, Lawrence E. Gibson, HeinzKutzner

• Skin

Tammie Ferringer

• Application of Direct Immunofluorescence for Skin and Mucosal Biopsies: A Practical Review

William B. Tyler

• Application of Fluorescent In Situ Hybridization (FISH) in Surgical and Cytologic Specimens (Solid Tumors, Hematopoietic Tumors, Urine, Bile Duct Brushing, and Bronchoscopy)

Hong Yin, Yi Ding, William Quinones, MicheleKay Zelonis, Dana Snyder

• Back Matter